Fluorescence Studies of the Interaction of Nucleotides with the Active Site of the Nuclease of Staphylococcus aureus

Digital Record

Identifier: 101584571X150

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Digital Object (text)

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Dates

Publication: November 1967

Extent

8 pages

Creator

Anfinsen, Christian B. (Christian Boehmer), 1916-1995 (Creator, Person)

Description

In the late 1960s, Anfinsen's lab sought to understand the complicated details of the physicochemical properties of Staphylococcus aureus in solution, ultimately to better understand the relationship of its structure to its function. In this article, Anfinsen, Cuatrecasas, and Edelhoch reported that the specific binding of a competitive inhibitor resulted in a marked decrease in tyrosyl fluorescence, which they interpreted as indicative of a specific interaction of the nucleotide with several tyrosyl groups in the active site of the enzyme.

Language of Materials

English

Additional Description

Original Profiles System Identifier

KKBBKF

Source Category

Periodical

Cuatrecasas, Pedro, Harold Edelhoch, and Christian B. Anfinsen. "Fluorescence Studies of the Interaction of Nucleotides with the Active Site of the Nuclease of Staphylococcus aureus." Proceedings of the National Academy of Sciences of the United States of America 58, 5 (November 1967): 2043-2050. Article. 8 Images.

Proceedings of the National Academy of Sciences of the United States of America