Studies of the Aromatic Circular Dichroism of Staphylococcal Nuclease

In this article, Anfinsen, Omenn, and Cuatrecasas described the circular dichroism spectra of nuclease and of performic acid-oxidized derivative. Their findings suggested that the binding of the nucleotide inhibitor altered the asymmetric environment of certain tyrosyl residues. Furthermore, through titration, a method which can determine a substance or component in a solution by the addition of a liquid reagent of a known strength until a given endpoint, they were subsequently able to determine with greater certainty several of the amino acids present in the amino chain for staphylococcal nuclease.