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Studies on the Reduction and Re-formation of Protein Disulfide Bonds

 Digital Record
Identifier: 101584571X142


  • Publication: May 1961


3 Pages



The information contained in the amino acid sequence of a protein is linear, such as it is in DNA, but proteins function by virtue of the arrangement of their linear structure into a well-defined three-dimensional conformation. In this article, Anfinsen and Haber sought to determine how the two-dimensional data of DNA were translated into the three-dimensional information that was characteristic of globular proteins. This simple and elegant experiment was designed to determine what would happen when reduced, unfolded, and enzymatically inactive ribonuclease was allowed simply to refold in the presence of only neutral aqueous buffer and air. They found that most of the activity of the enzyme was regained.

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Anfinsen, Christian B., and Edgar Haber. "Studies on the Reduction and Re-formation of Protein Disulfide Bonds." Journal of Biological Chemistry 236, 5 (May 1961): 1361-1363. Article. 3 Images.

Journal of Biological Chemistry

Physical Description

Physical Condition - Good

Collecting Area Details

Part of the Archives and Modern Manuscripts Collection Collecting Area

8600 Rockville Pike
Bldg 38/1E-21, MSC 3819
Bethesda MD 20894 US
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