Skip to main content

alpha-Amylase from the Hyperthermophilic Archaebacterium Pyrococcus furiosus

 Digital Record
Identifier: 101584571X158

Dates

  • Publication: 15 November 1993

Extent

6 Pages

Description

In this article, Anfinsen, et al, reported that while trying to study the factors that influenced protein thermostability, or the ability to remain stable at relatively high temperatures, they had cloned a gene from the hyperthermophilic bacterium Pyrococcus furiosus encoded with a highly thermostable alpha-amylase and expressed it in Escherichia Coli. The alpha-amylase expressed in E. Coli exhibited the temperature-dependent activation characteristic of the original enzyme from P. furiosus, but had a higher apparent molecular weight that was attributed to the improper formation of the native quaternary structure.

Language of Materials

English

Original Profiles System Identifier

KKBBKP

Source Category

Periodical

Laderman, Kenneth A., Kiyozo Asada, T. Uemori, H. Mukai, Y. Taguchi, I. Kato, and Christian B. Anfinsen. "alpha-Amylase from the Hyperthermophilic Archaebacterium Pyrococcus furiosus." Journal of Biological Chemistry 268, 32 (15 November 1993): 24402-24407. Article. 6 Images.

Journal of Biological Chemistry

Physical Description

Physical Condition - Good

Collecting Area Details

Part of the Archives and Modern Manuscripts Collection Collecting Area

Contact:
8600 Rockville Pike
Bldg 38/1E-21, MSC 3819
Bethesda MD 20894 US
1-888-FINDNLM (1-888-346-3656)