Skip to main content

The Kinetics of Formation of Native Ribonuclease During Oxidation of the Reduced Polypeptide Chain

 Digital Record
Identifier: 101584571X167

Dates

  • Publication: 15 September 1961

Extent

6 Pages

Description

In this article, Anfinsen, Haber, Sela, and White reported that there existed a considerable lag phase before enzymatic activity appeared after the sample of bovine pancreatic ribonuclease was treated with mercaptoethanol in urea, during which period the sulfhydrl titer and the specific optical rotation changed along a curve similar to that of a first-order reaction. The lag in enzymatic activity in vitro often took several hours, while the same process seemed to take only a few minutes in vivo. This discrepancy eventually led to the discovery of an enzyme system in the endoplasmic reticulum of cells that catalyzes the disulfide interchange reaction, and subsequently, the rapid formation of the correct, native disulfide pairing in relatively short order.

Language of Materials

English

Original Profiles System Identifier

KKBBKZ

Source Category

Periodical

Anfinsen, Christian B., Edgar Haber, Michael Sela, and F. H. White Jr. "The Kinetics of Formation of Native Ribonuclease During Oxidation of the Reduced Polypeptide Chain." Proceedings of the National Academy of Sciences of the United States of America 47, 9 (15 September 1961): 1309-1314. Article. 6 Images.

Proceedings of the National Academy of Sciences of the United States of America

Physical Description

Physical Condition - Good

Photocopy Only

Collecting Area Details

Part of the Archives and Modern Manuscripts Collection Collecting Area

Contact:
8600 Rockville Pike
Bldg 38/1E-21, MSC 3819
Bethesda MD 20894 US
1-888-FINDNLM (1-888-346-3656)