Solid Phase Synthesis of a 42-Residue Fragment of Staphylococcal Nuclease: Properties of a Semisynthetic Enzyme

Digital Record

Identifier: 101584571X173

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Digital Object (text)

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Dates

Publication: October 1969

Extent

8 pages

Creator

Anfinsen, Christian B. (Christian Boehmer), 1916-1995 (Creator, Person)

Description

After discovering an error in their original primary sequence analysis of staphylococcal nuclease, in which residue 43 was misidentified as glutamine instead of glutamic acid, Ontjes and Anfinsen published this paper. They reported that a resynthesis of the correct sequence in turn yielded a product with high biological activity. Therefore, they concluded that residue 43, although not essential for folding, was very important for catalytic activity once folding had occurred.

Language of Materials

English

Additional Description

Original Profiles System Identifier

KKBBLH

Source Category

Periodical

Ontjes, David, and Christian B. Anfinsen. "Solid Phase Synthesis of a 42-Residue Fragment of Staphylococcal Nuclease: Properties of a Semisynthetic Enzyme." Proceedings of the National Academy of Sciences of the United States of America 64, 2 (October 1969): 428-435. Article. 8 Images.

Proceedings of the National Academy of Sciences of the United States of America