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Human Lymphoblastoid Interferon: Purification, Amino Acid Composition, and Amino-Terminal Sequence

 Digital Record
Identifier: 101584571X177

Dates

  • Publication: 1980

Extent

9 Pages

Description

This article was the result of collaboration between various members of Anfinsen's lab at the NIAMD with Hunkapiller and Hood at Caltech. In it, they addressed the difficult problem of determining the amino acid sequence of Human Lymphoblastoid Interferon (HuIFN-alpha). Earlier attempts to sequence the amino-terminal portion of this molecule using conventional methods failed due to insufficient quantities of interferon. Through this collaboration, Anfinsen's lab developed a microsequencer, a technology that allowed them to obtain amino acid sequence data on 10 to 20 picomoles of protein. Because of this, they were able to successfully determine the amino-terminal amino acid sequence of the major component of human lymphoblastoid interferon (HuIFN-alpha).

Language of Materials

English

Original Profiles System Identifier

KKBBLM

Source Category

Periodical

Zoon, Kathryn C., Mark E. Smith, Pamela J. Bridgen, Dorothy Zur Nedden, Dorothea M. Miller, and Christian B. Anfinsen. "Human Lymphoblastoid Interferon: Purification, Amino Acid Composition, and Amino-Terminal Sequence." Annals of the New York Academy of Sciences 350, (1980): 390-398. Article. 9 Images.

Annals of the New York Academy of Sciences

Physical Description

Physical Condition - Good

Photocopy Only

Collecting Area Details

Part of the Archives and Modern Manuscripts Collection Collecting Area

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